FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9011072
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1215831.3492623297205928No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC784420.8705068608929105No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG526090.5838262084688702No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA388040.43062579013906443No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT361450.4011176472677169No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC355630.39465892626315713No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG223800.2483611272887399No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC199980.22192698049688206No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC179620.19933255443969375No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA153450.17029050483671643No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA138850.15408821503146353No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT138560.1537663887271126No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA137200.15225713433429452No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA134060.14877253228028806No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA132640.14719669313484565No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC132180.14668621003139248No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT113020.1254234790266907No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG110620.12276008892171764No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT108420.12031864799215898No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT99200.11008679100555406No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG98860.10970947740734954No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC97820.10855534169519454No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA96010.106546701657694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT706250.069.5937962
GTAGGTT675550.069.488638
CGGAGTC706600.069.4656114
AGTAGGT677450.069.4229057
TAGGTTG676200.069.385619
AAGATGC267250.069.378165
TGCTGGC267700.069.368829
CCACCGG709550.069.350210
GCTGGCA268100.069.31753510
AGGTTGT704100.069.3153710
AGCTTAT514750.069.308492
TAGTAGG678200.069.299696
TGAGGTA942050.069.2913741
GGTAGTA927250.069.287854
AGATGCT267550.069.276896
GGAGTCT707100.069.25165615
CAAGATG267750.069.222464
CACCGGA711000.069.19411
AGGTAGT927300.069.191293
GTTGTAT512550.069.1906112
Produced by FastQC (version 0.11.5)