FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10660725
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1363651.2791343928297558No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC882260.827579737775808No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG515520.48356936324687116No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA417160.39130546937473765No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT394430.3699842177713054No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC392740.36839895973303877No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA370620.3476499018593951No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA251210.23564063419701753No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG228890.21470397182180387No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC203390.19078439787162693No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC163720.1535730449852144No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT144580.13561929418496396No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA142810.13395899434606934No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA126180.11835968004052257No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA123970.1162866502981739No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT116610.10938280464039733No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA108620.10188800480267525No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG106750.1001339027129956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT825550.069.574732
AAGATGC336950.069.516315
AGATGCT337150.069.508176
CGGAGTC827350.069.4975514
TGCTGGC337850.069.447039
GCTGGCA337850.069.4366710
GTAGGTT860200.069.406118
GGAGTCT827150.069.3916515
TGAGGTA1187600.069.3663251
AGTAGGT863000.069.355317
CCACCGG831150.069.3433810
TAGGTTG861200.069.333649
CAAGATG337550.069.3305364
CTTCATT828400.069.3285453
AGGTTGT889150.069.3111710
GGTAGTA1173250.069.269244
TAGTAGG863100.069.2621156
AGGTAGT1173700.069.2237553
GTTGTGT248750.069.1662812
TATAGTT763600.069.1487616
Produced by FastQC (version 0.11.5)