FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10007447
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1172771.1718972880895597No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1048501.047719763092425No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA584360.583925150940095No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG455070.45473136155504995No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC446400.44606781329943596No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA372270.3719929768301546No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA370040.3697646362753657No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT349570.34930986894059995No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG266850.2666514246840378No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC255060.2548701981634277No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT194950.19480492876954533No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC164510.16438758056875044No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA160120.16000084736896433No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC154270.15415520062209673No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA135420.13531922777107888No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA134610.1345098305292049No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT131620.13152205552525034No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA123030.12293844773796953No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA120700.12061018159776414No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG119560.11947102992401559No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC110880.11079748910985988No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG102040.10196406735903774No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT702400.069.6061252
TGCTGGC281900.069.498179
CGGAGTC703300.069.4765414
GTAGGTT780300.069.4520658
AGATGCT282000.069.411826
AGTAGGT782400.069.399847
TGAGGTA1077300.069.3339841
AGGTTGT808150.069.3105310
TAGGTTG781550.069.309639
GGTAGTA1064150.069.295464
GCTGGCA282500.069.2638410
CAAGATG282800.069.262544
CTTCATT705500.069.254933
TATAGTT698000.069.2518216
AAGATGC282900.069.250435
TAGTAGG783100.069.2487646
GTTGTAT595950.069.2202712
GGAGTCT705000.069.1986415
AGCTTAT505600.069.1571962
CCACCGG708300.069.144910
Produced by FastQC (version 0.11.5)