FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10529955
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1446131.3733486990210308No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA981360.9319697947427127No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG891220.8463663899798243No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC743090.7056915247975893No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA687500.6528992763976674No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG621480.5902019524299962No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC372780.3540186069171236No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG344930.3275702507750508No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA282620.26839620872074005No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC263180.24993459136340088No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT261660.24849109041776532No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA257140.24419857444784904No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG248390.2358889472936969No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT246190.23379966960922435No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA234920.2230968698346764No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA219260.208225011407931No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA188530.1790416008425487No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG185660.1763160431359868No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC141350.1342360912273604No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT124680.11840506440910716No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA120490.11442594009186174No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC113700.10797766942023969No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC112340.10668611594256575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT626650.069.527742
AGATGCT255950.069.5163046
AGTAGGT662050.069.466267
AAGATGC256400.069.448575
GTAGGTT661550.069.423548
TAGGTTG662050.069.3555769
TGAGGTA893700.069.271141
TGCTGGC256450.069.258279
AGGTTGT686150.069.2556210
TAGTAGG663400.069.251046
CAAGATG257200.069.246174
GCTGGCA256550.069.20410
GGAGTCT626050.069.1997615
AGCTTAT418700.069.177712
GTTGTAT494100.069.173912
GGTAGTA880150.069.163514
CTTCATT630150.069.06873
TATAGTT569000.069.02798516
TTCATTC631000.068.957394
TGTATAG624050.068.9372414
Produced by FastQC (version 0.11.5)