FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9562876
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG732520.7660038674557738No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA692140.7237780768045095No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC479830.5017632770727133No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA454230.47499308785348676No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG295740.3092584281130488No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG285980.2990522934732187No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC274010.2865351385921976No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA228600.2390494240435618No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT218810.2288119180882404No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC175320.1833339677310466No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA166880.17450817097283286No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA129150.13505351318996503No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC120500.12600811722331232No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC108730.11370010444556637No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG105940.11078257210487723No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA97100.10153849114011308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATGC207150.069.502465
CCTTCAT497200.069.4733352
AGATGCT207650.069.302116
GTAGGTT527700.069.290138
TGCTGGC208050.069.286629
CTTCATT497900.069.276543
GGAGTCT496400.069.2438315
TGAGGTA716600.069.220051
TAGGTTG528000.069.217629
AGGTTGT547700.069.2007910
AGTAGGT529700.069.160657
TAGTAGG529250.069.1268846
CAAGATG208300.069.118744
TTCATTC498850.069.073374
GTTGTAT400950.069.0602612
GCTGGCA208950.068.9881810
GCATAGC206350.068.9769714
TATAGTT463100.068.9646516
AGCTTAT302950.068.908322
GATGCTG209350.068.873097
Produced by FastQC (version 0.11.5)