FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6862116
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC674700.9832244164919393No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA560560.8168908832202778No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG398740.5810744091181205No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA355730.5183969492792019No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC334910.4880564537236036No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG193400.2818372641908123No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC162520.23683656761267227No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG144970.2112613660276218No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC136030.1982333146219038No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA126360.18414145141236318No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA124420.1813143351117935No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA115570.16841743858599884No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT113180.16493454788581247No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA109700.1598632258621102No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT103610.15098841232063112No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA99290.14469297808431103No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG82500.12022530659639095No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG78290.11409017276886606No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA76560.11156908452145081No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC69700.10157216811840546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT228750.069.230232
CCTTCAT291550.069.2269442
TGAGGTA353050.069.1090851
GTAGGTT253150.069.095088
AAGATGC107600.069.081475
GCTTATC229150.069.076833
AGGTTGT260500.069.0532610
AGATGCT107500.069.017016
TAGGTTG253300.069.012719
CTTCATT292300.068.999423
AGTAGGT254000.068.987867
TGCTGGC107700.068.921359
GTTGTAT191600.068.8977412
GTTGACA157550.068.8905518
GGAGTCT292300.068.8757515
TTCATTC292700.068.832394
TATAGTT229650.068.7210916
TAGTAGG255150.068.663216
CAGACTG231000.068.524119
TCATTCC294650.068.483745
Produced by FastQC (version 0.11.5)