FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9039169
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1403151.5522997744593556No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG928921.0276608391766986No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC729610.8071649064200482No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA595110.6583680424605404No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG400230.4427730026952699No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA384870.42578029020145547No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC341720.3780436011319182No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA306200.33874795348997233No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT297240.3288355378685806No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA260510.28820127159919234No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG258750.2862541899592761No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA234550.25948181741042786No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT219950.24332989017021364No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG182390.20177739790018306No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC177050.19586977519725543No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC135090.14944957882743426No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA128850.14254628937682215No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT128780.14246884862978001No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA117310.129779629078735No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA106800.1181524540585534No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG105910.11716785027473212No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG100120.11076239419796222No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC91220.10091635635974944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT640900.069.604432
CGGAGTC641450.069.4812614
AAGATGC252300.069.480515
TGCTGGC252600.069.455339
AGATGCT253000.069.331696
GTAGGTT609650.069.329768
GGAGTCT641450.069.31711615
CCACCGG645050.069.29034410
CAAGATG253000.069.288284
AGTAGGT611300.069.2628567
GGTAGTA837300.069.262284
CTTCATT643550.069.256843
GCTGGCA253650.069.250610
TGAGGTA849250.069.248811
TAGGTTG610300.069.2387169
AGCTTAT498600.069.211432
TAGTAGG611050.069.18816
AGGTTGT631400.069.1476110
TTCATTC645100.069.0660554
GTTGTAT465900.069.04166412
Produced by FastQC (version 0.11.5)