FastQCFastQC Report
Sun 12 Aug 2018
HGKMWBGX7_n01_mycscreens2_5_bc_bc24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_mycscreens2_5_bc_bc24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24898417
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG4568750.069.9568251
TAACAAT4941750.069.779151
TCGATAC4583350.069.771484
TAGGTCG5586450.069.758993
AGGTCGC5588750.069.7175454
ATAGGTC5595350.069.6968842
TACTGTA4815800.069.658182
GGTCGCA5595900.069.621045
TCGATAG4594100.069.596725
CTGTATC4821750.069.583364
ACTGTAT4822800.069.5692443
GTCGCAT5600550.069.5696
TCGCATC5604600.069.542497
GTATCTC4834000.069.4534766
TGTATCT4834400.069.442525
TATCTCT4844300.069.397057
ATACTGT4837600.069.366921
AACAATG4987700.069.355692
ATCTCTT4847850.069.336548
CGATAGG4613850.069.2443166
Produced by FastQC (version 0.11.2)