FastQCFastQC Report
Sun 12 Aug 2018
HGKMWBGX7_n01_mycscreens2_5_bc_bc21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_mycscreens2_5_bc_bc21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27353486
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG5265100.069.956021
TAGGTCG6739700.069.763283
TAACAAT5379000.069.750741
TCGATAC4272900.069.739014
AGGTCGC6740850.069.711784
ATAGGTC6750000.069.710892
TCGATAG5293200.069.644665
TACTGTA5261250.069.644262
GGTCGCA6747800.069.633885
CTGTATC5266500.069.60354
GTCGCAT6752400.069.580366
TCGCATC6755550.069.5749367
ACTGTAT5267250.069.565973
GTATCTC5279600.069.492056
TGTATCT5281700.069.4676065
TATCTCT5290600.069.429667
AACAATG5423000.069.376772
ATCTCTT5294600.069.35148
ATACTGT5285050.069.3331
CGATAGG5318200.069.259486
Produced by FastQC (version 0.11.2)