FastQCFastQC Report
Sun 12 Aug 2018
HGKMWBGX7_n01_mycscreens2_5_bc_bc20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_mycscreens2_5_bc_bc20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21639696
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTCCTCAGCATCTTAT463000.2139586434116265No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGTCCTCAGCATCTTATC432110.19968395119783566No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTCCTCAGCATCTTATCC426730.19719777948821465No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTCCTCAGCA420520.19432805340703493No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTCCTCAGCAT409480.18922631815160434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG4192350.069.9615251
TAACAAT4277600.069.767621
TAGGTCG4750800.069.765653
TCGATAC3970200.069.7335744
AGGTCGC4752300.069.727014
ATAGGTC4762400.069.675342
TCGATAG4220200.069.636875
GGTCGCA4758100.069.633355
TACTGTA4097200.069.603572
GTCGCAT4763050.069.5884866
TCGCATC4765550.069.5642857
CTGTATC4101650.069.530564
ACTGTAT4104450.069.487923
TGTATCT4112600.069.421345
GTATCTC4111600.069.413016
ATACTGT4110850.069.3851241
AACAATG4313950.069.3720552
TATCTCT4118000.069.3514257
ATCTCTT4121450.069.250398
CGATAGG4239600.069.2450566
Produced by FastQC (version 0.11.2)