FastQCFastQC Report
Sun 12 Aug 2018
HGKMWBGX7_n01_mycscreens2_5_bc_bc17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_mycscreens2_5_bc_bc17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20764724
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC504390.24290715349744113No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG477670.2300391760564696No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG476520.22948535217708646No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG473630.22809356868889757No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC398860.19208538480935264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG4171500.069.955491
TAGGTCG4439800.069.7905043
TAACAAT4279750.069.771881
AGGTCGC4440350.069.75054
ATAGGTC4448750.069.7182162
GGTCGCA4445700.069.643455
TACTGTA4254400.069.632772
TCGATAC3285150.069.621824
TCGATAG4194100.069.6100855
GTCGCAT4449800.069.5984956
CTGTATC4257200.069.596524
TCGCATC4452150.069.574817
ACTGTAT4259150.069.562843
GTATCTC4266900.069.512156
TGTATCT4266650.069.498665
TATCTCT4276750.069.4313357
ATACTGT4268350.069.424391
AACAATG4317650.069.374742
ATCTCTT4280050.069.33978
AATGGTC4319300.069.267395
Produced by FastQC (version 0.11.2)