FastQCFastQC Report
Sun 12 Aug 2018
HGKMWBGX7_n01_mycscreens2_5_bc_bc16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_mycscreens2_5_bc_bc16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22477290
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT389700.17337499315976257No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT370690.1649175679096546No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC358620.1595477034820479No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA357040.15884477176741502No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC351080.15619320656538221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG4449750.069.9577561
TAACAAT4319800.069.773211
TAGGTCG4899000.069.765543
TCGATAC4186400.069.7561654
AGGTCGC4900850.069.7073754
ATAGGTC4910850.069.690412
TCGATAG4473500.069.661625
GGTCGCA4906950.069.617065
TACTGTA4258100.069.616032
CTGTATC4261300.069.578184
GTCGCAT4910700.069.5645146
TCGCATC4913850.069.542487
ACTGTAT4263450.069.534583
GTATCTC4274850.069.455496
TGTATCT4275400.069.434485
AACAATG4357600.069.394362
ATACTGT4274800.069.370381
TATCTCT4285250.069.367697
ATCTCTT4287050.069.298368
CGATAGG4494400.069.2681966
Produced by FastQC (version 0.11.2)