FastQCFastQC Report
Sun 12 Aug 2018
HGKMWBGX7_n01_mycscreens2_5_bc_bc13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_mycscreens2_5_bc_bc13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18545116
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT790420.42621464325162484No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC758020.4087437360866333No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA745730.40211665432559174No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT731660.39452975112153515No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC686760.37031852483424743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG3578300.069.954641
TAACAAT3697400.069.775441
TAGGTCG4003250.069.749663
TCGATAC3714200.069.74334
AGGTCGC4003450.069.717514
ATAGGTC4012000.069.698552
GGTCGCA4007900.069.6340565
TCGATAG3601250.069.617515
TACTGTA3242000.069.6165162
GTCGCAT4009500.069.596666
TCGCATC4013400.069.5677647
ACTGTAT3247000.069.524593
CTGTATC3248850.069.499194
AACAATG3727650.069.407712
GTATCTC3257300.069.393986
TGTATCT3257450.069.388645
TATCTCT3266250.069.298647
ATACTGT3257500.069.292131
ATCTCTT3269100.069.253778
CGATAGG3618050.069.242976
Produced by FastQC (version 0.11.2)