FastQCFastQC Report
Sun 12 Aug 2018
HGKMWBGX7_n01_mycscreens2_5_bc_bc12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_mycscreens2_5_bc_bc12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19043178
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG4003300.069.961671
TCGATTC4092650.069.852082
ATCGATT4095900.069.804531
TAACAAT3806850.069.7765351
TCGATAA3569600.069.700033
TCGATAG4024300.069.666765
ATTCCTT4093200.069.6425255
TCGATAC3286650.069.575194
GATAACG3573100.069.574085
ATAACGC3571850.069.557396
TAACGCA3572800.069.526637
CGATTCC4104250.069.499993
AACGCAT3576000.069.454998
CGATAAC3580300.069.4529954
AACAATG3837150.069.441552
ACGCATT3577400.069.433689
TTGGTTC4092700.069.4116510
TTCCTTG4104450.069.405846
GATTCCT4112950.069.377174
CGATAGG4041300.069.31336
Produced by FastQC (version 0.11.2)