FastQCFastQC Report
Sun 12 Aug 2018
HGKMWBGX7_n01_mycscreens2_5_bc_bc09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_mycscreens2_5_bc_bc09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21782658
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG3246250.069.952651
TCGATTC4840800.069.879582
ATCGATT4844500.069.8216251
TCGATAA6928050.069.801683
TAACAAT3399000.069.701561
GATAACG6929750.069.69095
ATAACGC6929550.069.668836
TAACGCA6929500.069.647637
CGATAAC6938400.069.6450654
ATTCCTT4846150.069.632545
TCGATAG3264800.069.589235
AACGCAT6936350.069.570398
CGATTCC4854050.069.549423
ACGCATT6941650.069.527519
TTGGTTC4844300.069.4281810
TCGATAC3077200.069.405574
GATTCCT4866700.069.4029164
TTCCTTG4860950.069.402686
GGTTCTT4848700.069.3004512
CGCATTT6959800.069.2910410
Produced by FastQC (version 0.11.2)