FastQCFastQC Report
Sun 12 Aug 2018
HGKMWBGX7_n01_mycscreens2_5_bc_bc08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_mycscreens2_5_bc_bc08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27856760
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTCCAGC492000.17661781197813384No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTCCAG281560.1010742096352914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACG6313250.069.906281
TACACGA6311500.069.879912
GATCGCG1560650.069.850761
CGATCGT3851200.069.835092
ATCGCGC1561150.069.8292542
TCGATCG3853900.069.803891
TAAGTAG12275400.069.794791
ACACGAT6321350.069.792623
ATCGTTA3845850.069.771394
CACGATC6323900.069.755324
TCGTTAC3845550.069.754045
ACGATCT6331800.069.7238855
TCGCGCG1564200.069.6572953
AAGTAGA12316350.069.579552
TAGAGTC12325550.069.5443655
GATCGTT3865350.069.509723
CGATCAT3404650.069.494561
GTAGAGT12331850.069.484684
ATGATCG3414800.069.482886
GATCGTC3414150.069.4512948
Produced by FastQC (version 0.11.2)