FastQCFastQC Report
Sun 12 Aug 2018
HGKMWBGX7_n01_mycscreens2_5_bc_bc01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_mycscreens2_5_bc_bc01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26488314
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTCCAG1764600.6661805655127767No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG1208710.4563182088524019No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTCCAGC1152010.43491254294252174No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG765960.2891690275190788No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTCCA672760.25398370013282084No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC457090.17256288943116577No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTC376990.1423231391775256No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTCC335890.1268068628301522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG2194200.069.9635851
ATACACG11117850.069.9445951
TACACGA11118500.069.9251862
GATCGCG3943500.069.896071
ACACGAT11134250.069.856543
ATCGCGC3944850.069.855662
CACGATC11136800.069.830564
ACGATCT11152400.069.788025
TCGCGCG3949950.069.774723
CGATCGT2200800.069.748482
ATCGTTA2195050.069.729464
TCGTTAC2196150.069.679135
TAAGTAG6887900.069.659451
CGATCTC11172350.069.647096
GATCTCT11189900.069.574027
GCGCGGT3952450.069.560585
ATCTCTT11236450.069.5593648
TCTCTTG11241700.069.4908149
GCGGTTC3954900.069.479667
CTCTTGT11224550.069.4719810
Produced by FastQC (version 0.11.2)