FastQCFastQC Report
Tue 25 Oct 2022
HGKL5DRX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKL5DRX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000759972
Sequences flagged as poor quality0
Sequence length10
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAACATTC95928257195.85540967260029No Hit
AGATCTCGGT145393351.4528293903425626Illumina Single End PCR Primer 1 (100% over 10bp)
CGATCTCGGT96202990.9612993394184236No Hit
TGATCTCGGT30281170.3025817463450666No Hit
NACAACATTC20204000.20188657185821177No Hit
GGGGGGGGGG10834300.1082607248803912No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)