FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13605683
Sequences flagged as poor quality0
Sequence length76
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG249660.18349685201397092No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG183610.13495096130050951No Hit
GCGACCCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG175280.12882851967078757No Hit
CGACCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140590.10333182097510282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGG455850.067.0040366
GTCGCGG177550.065.272175
TTCGCGG136150.064.970745
CGTGGGG636350.063.0362747
ACGCGGG170400.062.15866
CGCTGGG68550.061.2525567
CTCGCGG74550.061.0174565
TGTCGCG64300.060.9515274
ATCGCGG98800.060.7032975
GGATAGT98000.060.3419046
CCGTGGG128100.059.9848566
CGCGGGG1146750.059.8080867
CTCGGGG67350.059.5395285
GGGATAG96750.059.5301865
ACGTGGG91500.059.465866
GCGTGGG266200.058.967086
GATCGCG40500.058.0619744
GGATAGG96600.058.0288246
TCGTGGG198000.057.8944746
TGGATAG95700.057.806725
Produced by FastQC (version 0.11.5)