FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14685915
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG178300.064.4251566
GGATAGT189150.063.4311756
GGGATAG185650.062.5532235
GATAGGT157400.062.23877
CTGATAG78450.061.5679055
TGGATAG172000.061.5349125
GGGGATA187250.061.0654534
CGGATAG78550.060.865725
GTATAGG145400.060.660286
CGTATAG59750.060.1590235
CTATAGG67400.060.02976
CGATAGT81350.059.2870566
GGTATAG141400.059.257455
CAGATAG68950.059.2386055
GGGTATA158450.059.02184
GCGATAG80000.058.975035
CGTATAC23650.058.752675
GATAGGG161650.058.5887767
GGATATG231650.058.5474056
GATAGTC68600.058.468627
Produced by FastQC (version 0.11.5)