FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14842716
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG216050.062.709316
GGGATAG226300.062.374465
GGATAGT227000.061.827496
CGGATAG77800.060.462045
GATAGGT203550.060.1806837
GGGGATA231550.059.5847244
TGGATAG215800.058.824635
ATAGGGG118300.057.8981678
GATAGGG206150.057.3673827
GCGATAG88850.056.6453865
TGATAGG163200.056.4025426
AGGATAG107200.056.188735
CGGGATA117400.056.0470624
CTGATAG79550.055.9201665
GGATATG288800.055.759416
GTCGATA83850.055.515174
CGATAGT92100.055.406546
GGTATAG134450.055.3173335
GTATAGG133650.054.731896
GGGTATA150350.054.658514
Produced by FastQC (version 0.11.5)