FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13833351
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG171800.063.6221966
GGATAGT185300.062.537946
GGGATAG182550.062.4063845
TGGATAG169050.061.2617845
GATAGGT159600.061.161127
CGGATAG73050.060.7996675
GGGGATA179300.060.785244
CGTATAG55650.059.6215135
GTATAGG131650.059.3114556
CTGATAG71600.058.8047565
GGTATAG130500.058.7881625
CGGGATA102450.058.4859624
GCGATAG77350.058.4604345
CGATAGT78750.058.3988956
GGGTATA147600.058.189964
TGATAGG132150.058.107116
GCGGATA85250.057.394814
GATAGTC73650.057.3105247
GGATATG224800.057.200876
CTATAGG58550.056.8477756
Produced by FastQC (version 0.11.5)