FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26915274
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG136650.063.647375
GGATAGT365500.063.5068936
GGATAGG323400.063.343536
GGGATAG366350.061.8309635
GGGGATA347200.061.7937364
GATAGGT311400.061.5354277
TGGATAG331150.060.772465
GGTATAG257550.060.473095
CGTATAG107800.060.129275
GGGTATA283750.059.5888334
GTATAGG256300.059.5799756
CGCTACC18050.059.5284966
CTATAGG109100.058.7711836
CTGATAG130800.058.6270875
ATAGGGG183800.058.5731938
CGGGATA199400.058.4146654
GCGGATA169650.058.157394
CGGTATA150000.057.866094
GGATATG444150.057.5644386
GGTATAA245900.057.4455385