FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16332240
Sequences flagged as poor quality0
Sequence length76
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG174250.063.591936
GGATAGT195250.063.2593236
GGGATAG187550.062.7400055
GATAGGT170750.062.0661937
GGGGATA193350.061.744954
TGGATAG174200.060.9982765
CGGATAG79450.060.0875325
CGTATAG62850.059.864195
GATAATT550400.059.5128757
CAGATAG72600.059.393375
CTGATAG83500.059.3527265
GGATATG257900.059.318946
CTATAGG68550.059.2773326
GATAGGG149350.059.1719747
CGGGATA121650.058.836264
GTATAGG144550.058.5950856
TGATAGG141700.058.291616
CGATAGT86550.058.2316786
AGGATAG93900.058.183685
GGTATAG144350.058.045865
Produced by FastQC (version 0.11.5)