FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29899
Sequences flagged as poor quality0
Sequence length76
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAG252.773746E-670.000016
TATAGTG252.773746E-670.000017
AGGGGTT150.00220367770.010
CTATAGT150.00220367770.06
CCTATAT207.8029894E-570.05
CTATACG150.00220367770.06
TATACGG150.00220367770.07
TATACGC150.00220367770.07
TATACAC207.8029894E-570.07
ATAAGGT150.00220367770.08
CACGTTA150.00220367770.059
GCGATAT150.00220367770.05
CAGTATA150.00220367770.04
AGGATAG353.5506673E-970.05
ATAGGGG207.8029894E-570.08
TATAAGC207.8029894E-570.07
AGGGTGA150.00220367770.02
TACACGA150.00220367770.09
GGATAGG353.5506673E-970.06
GTATACA150.00220367770.06
Produced by FastQC (version 0.11.5)