FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54153
Sequences flagged as poor quality0
Sequence length76
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTG252.8045797E-670.000017
ATAGGTA252.8045797E-670.000018
ACTGATA252.8045797E-670.000014
CTATAGG252.8045797E-670.000016
GGATAGG500.070.000016
GGATAAG252.8045797E-670.000016
ATACTGT252.8045797E-670.000018
GCCTATA252.8045797E-670.000014
ATAAAGG252.8045797E-670.000018
GATAGGT252.8045797E-670.000017
GTCGATA207.860697E-570.04
CGTATAG301.0059557E-770.05
ATAGGCC150.00221344270.08
CGTTGTA150.00221344270.02
TAGGTGG150.00221344270.09
CTATACC207.860697E-570.06
GTGATAG401.3096724E-1070.05
CGTCTAC150.00221344270.05
GTCTACC150.00221344270.06
ATAGACC207.860697E-570.08
Produced by FastQC (version 0.11.5)