FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64699
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1200.18547427317269202No Hit
GGGGGGCGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG690.10664770707429791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCT401.3096724E-1070.000018
CTATAGA207.872244E-570.000016
ATAGCTG207.872244E-570.000018
ATAGCGT207.872244E-570.000018
GGATAGC207.872244E-570.000016
GAGGTGA207.872244E-570.000012
GTATAGG550.070.000016
CGAGATA207.872244E-570.000014
CGATATG455.456968E-1270.000016
CGTATAG150.002215393170.05
ATAGGGA301.0090662E-770.08
ATAGGAG150.002215393170.08
TCCGATA150.002215393170.04
CCGATAA150.002215393170.05
TAGGTTC150.002215393170.09
ATTGTCG150.002215393170.010
TATATCC252.8107625E-670.07
ATATCCG150.002215393170.08
CGGATAG150.002215393170.05
CGGATAA252.8107625E-670.05
Produced by FastQC (version 0.11.5)