FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19682
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATC150.00219241470.06
CGATAAT150.00219241470.06
TGGTATA353.4669938E-970.04
TCGATAA150.00219241470.05
TGCGTAT150.00219241470.03
ATAGGTG150.00219241470.08
GATGTGT150.00219241470.09
GATAATG309.728683E-870.07
GTCGATA207.7366014E-570.04
GGCGATA150.00219241470.04
TTATAGT252.7383958E-670.06
TGATAGG150.00219241470.06
GGATATC150.00219241470.06
GTATAGG252.7383958E-670.06
GGATAGT353.4669938E-970.06
GGATAGG252.7383958E-670.06
GTATACT150.00219241470.06
GTATAAG207.7366014E-570.06
ATAGTTG207.7366014E-570.08
GGGGGAT207.7366014E-570.03
Produced by FastQC (version 0.11.5)