FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4692
Sequences flagged as poor quality0
Sequence length76
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATGG207.1288094E-570.07
ATAAGAT150.00208776670.08
TATAAGA150.00208776670.07
TTATAGA150.00208776670.06
CATTATA150.00208776670.04
GTATAAA150.00208776670.06
GTGATAT150.00208776670.05
TTTATAT207.1288094E-570.05
GATAGTT150.00208776670.07
GTGTATA307.14656E-658.3333324
ATAGGTT200.0065266852.58
TTATATG200.0065266852.56
ATAGTTG200.0065266852.58
TTTATAG200.0065266852.55
TGTATAA350.001131217940.05
TTATTTA400.00218082535.063
TGTGTAT500.00650734227.9999983
Produced by FastQC (version 0.11.5)