FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4264
Sequences flagged as poor quality0
Sequence length76
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTATA150.002075072870.04
TATAATC150.002075072870.07
TTTTTAT150.002075072870.03
TCAATAT150.002075072870.03
GCTATAA150.002075072870.05
GTTATAT307.0373862E-658.3333325
TTATATT252.1294119E-455.9999966
TATATGT200.006487296452.57
TTATATG200.006487296452.56
ATAATTG200.006487296452.58
CAATATA200.006487296452.54
TCTTATA200.006487296452.54
ATATATA200.006487296452.54
TTTTATA350.00111989740.0000044
TATAATT400.002159128235.07
GTAATAA400.002159128235.036
TGTAATA400.002159128235.035
TAATAAT500.00644335727.99999837
Produced by FastQC (version 0.11.5)