FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6382307
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG80910.12677234109860275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATAG85100.066.2563865
GGATAGG84450.066.06186
GTATAGG84550.066.025076
CTATAGG38000.065.5786
GGTATAG78800.065.513015
GATAGGT71450.065.345967
CGTATAG37750.064.807015
GGGTATA76550.064.512464
GGATAGT85750.064.448056
GGTATAA60150.064.413035
GGGGATA75700.064.0347444
CAGATAG27100.063.7998165
CCTATAG15250.063.5728575
GTATACG23350.063.5536846
GTATAGT88200.063.4117856
GGTATAC25250.063.345625
TGGATAG75750.063.345625
CGGGATA44000.063.158184
ATAGGTC25950.063.1209758
GTTATAG114450.062.996035
Produced by FastQC (version 0.11.5)