FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22921312
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT351350.064.261436
GATAGGT332550.063.221117
GGGATAG316500.063.154135
GGATAGG306300.063.0518726
CGGATAG109650.061.3815
GGGGATA334750.061.3212134
CGGGATA137800.061.135044
CGATAGG107200.060.6296
CTGATAG135150.060.598675
CGCGATA48950.060.2036364
GGTATAG212400.059.931085
GCGATAG109700.059.5663535
TGATAGG277750.059.5403026
AGGATAG145300.059.256075
GTGATAG303000.059.2105755
GCGATAA79000.059.1006245
CAGATAG84100.059.095675
TGGATAG335600.058.7672355
GTATAGG224900.058.669886
TGGGATA404950.058.1929744