FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16639615
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT228100.064.276716
GATAGGT236650.063.2398227
CGGATAG87850.062.9084435
GGATAGG224700.062.6636286
CGGGATA101300.061.8460734
CTGATAG109800.061.2022635
GGGGATA222200.061.0846824
GGGATAG222450.061.0160335
TGATAGG214900.060.6841136
CGCGATA40200.060.248834
GTATAGG158300.059.6305166
GTGATAG215800.059.344375
GCGATAG89500.059.1676675
GGTATAG143600.058.544645
TGGATAG229750.058.4832045
CAGATAG71700.058.4798475
AGGATAG102950.058.475065
GATAGTG207150.058.4251027
ATAGGTC57100.058.4137238
CGTGATA95500.058.345624
Produced by FastQC (version 0.11.5)