FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15033718
Sequences flagged as poor quality0
Sequence length76
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT230350.063.6643456
GATAGGT213550.062.918797
GGGATAG202300.062.318695
GGATAGG196050.061.7167476
CGGATAG69750.061.1688045
GGGGATA215300.060.4253234
CGGGATA86500.060.370284
CGCGATA33500.059.9704864
TGATAGG170850.059.6556
CGTGATA85350.059.0512964
GTGATAG187450.059.0400735
CAGATAG52050.059.0397155
CTGATAG82200.059.014935
GGTATAG133200.058.911745
GCGATAA52900.058.885025
CGATAGG68150.058.7017256
TGGGATA258250.058.616014
AGGATAG94100.058.4328425
TGGATAG223450.058.378045
GCGATAG66600.058.2285545
Produced by FastQC (version 0.11.5)