FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17775300
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT271400.064.260166
GATAGGT255000.063.9187
GGATAGG239550.063.7163246
CGGATAG84250.063.1858865
GGGATAG244250.063.04915
CGGGATA102450.061.9706574
GGGGATA262050.061.5444764
CGATAGG80650.060.7119456
CGCGATA38750.060.4247934
AGGATAG107650.060.0500875
GGTATAG161250.060.03625
GCGATAG83600.060.034885
GTGATAG240550.059.9158555
CTGATAG102450.059.75015
TGATAGG211900.059.5436026
CAGATAG64300.059.3302735
CGTGATA101800.059.134574
TGGATAG262700.058.9407775
GCGATAA63400.058.84765
GTATAGG174400.058.700286
Produced by FastQC (version 0.11.5)