FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16215660
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT235300.064.6741266
GATAGGT222850.064.2193767
GGATAGG215800.063.6577266
GGGATAG223300.063.1653985
CGGATAG78700.061.682885
CGGGATA95700.061.2949944
AGGATAG97800.061.0166665
GGGGATA233100.060.9602434
CGATAGG76300.060.733236
GTGATAG213650.060.7107285
TGATAGG195450.060.3292966
GCGATAA59350.060.0329865
CTGATAG95350.059.8681955
GGTATAG147850.059.7727175
CGCGATA35000.059.6992954
CAGATAG61050.059.50795
GTATAGG158800.059.309756
CGTGATA87150.059.196094
GATAGTG202550.058.387187
ATAGGGT150600.058.378778
Produced by FastQC (version 0.11.5)