FastQCFastQC Report
Fri 21 Dec 2018
HGKKNBGX9_n01_Lex-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKKNBGX9_n01_Lex-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43313861
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG596350.063.7075966
GGGATAG664450.063.1619765
GGATAGT669450.062.596136
GGGGATA668200.061.4613724
GATAGGT587300.061.149137
CGGATAG212100.060.4447945
TGGATAG613400.059.363465
GGTATAG441200.058.9249275
GTATAGG413850.058.133876
GGGTATA497850.057.72454
ATAGGGG337450.057.6666878
GATAGGG574400.057.4833537
GGATATG851550.057.319476
GGGATAA542700.057.197555
AGGATAG295250.057.184725
GCGATAG242000.057.1418465
CTGATAG211900.057.06635
CGGGATA344150.057.042734
CGGTATA245450.056.7379344
CGTATAG153400.056.7202845
Produced by FastQC (version 0.11.5)