FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38932568
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG613596815.760501593421733No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG394600.10135473211014491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC345250.048.95326613
CGTCTGA345000.047.58971816
ACGTCTG347950.047.4469915
CACGTCT355550.047.41650814
CACACGT362650.047.23159412
GCACACG366100.047.09223611
ATCGGAA455000.046.9283332
CGGAAGA441350.046.6510664
TCGGAAG452700.046.5322043
GATCGGA457250.046.445131
GAACTCC391800.039.5652721
AGCACAC450750.039.3742710
AGAGCAC478250.038.968828
CTGAACT429450.037.80444319
TGAACTC460650.035.0256920
TCTGAAC480750.034.25666418
AACTCCA449800.034.1839422
CGATCGA11400.034.06406433
GAGCACA550700.033.4161839
ACGATCG13100.033.38236232
Produced by FastQC (version 0.11.5)