FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_mb229.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_mb229.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22097064
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1343950.6082029721233554TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG196250.061.7867143
CTCGTAT191200.061.73464642
CGTATGC198000.061.68330844
CGGCTAC200850.061.3637833
ACGGCTA203100.061.30425332
TCTCGTA185200.061.03248641
ATGCCGT199950.060.48790747
TATGCCG203450.059.9109446
TGCCGTC201900.059.76543848
GCCGTCT200300.059.31724549
GTCACGG212900.059.239829
CCGTCTT201900.058.89983750
TCACGGC212850.058.7595530
CATCTCG186350.058.5523439
AGTCACG218650.057.90677628
CACGGCT215700.057.85325231
ATCTCGT189250.057.78454240
CGTCTGA224550.057.61374316
CGTCTTC207500.057.1249951
ACACGTC228000.056.8955713
Produced by FastQC (version 0.11.5)