FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_mb228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_mb228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28223578
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC3527511.2498450763400728TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT463450.064.8684442
TCGTATG466300.064.8105943
CGTATGC489900.064.7680944
TCTCGTA453550.064.7254941
GATCGGA595500.064.670981
ATCGGAA601750.064.266672
TATCTCG469450.064.2476339
ATGCCGT484450.064.15494547
ACACGTC592150.064.1534413
TATGCCG498750.063.98515746
CGTCTGA594550.063.8237316
ACGTCTG599500.063.48330715
TGCCGTC477950.063.3729848
ATCTCGT472650.062.6944640
CACGTCT608200.062.68441414
CCGTCTT464000.062.6470250
GCCGTCT457300.062.2865149
TCGGAAG626450.062.0452233
CGTCTTC452300.061.69936851
CGGAAGA630000.061.2900054
Produced by FastQC (version 0.11.5)