FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_mb227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_mb227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30181213
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC23376277.745305001492153TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGC1277090.42314071339677434TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATGC764190.2532005589039778TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC707940.23456313700844297TruSeq Adapter, Index 9 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG397190.13160173515888843No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATGTCGTATGC353100.11699330971223722TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG2772650.065.5176339
TCTCGTA2935250.065.4972441
CTCGTAT3053200.065.4302642
CGTATGC3240850.065.3918544
ATGCCGT3211950.065.3907847
TCGTATG3220550.065.3891743
CGATCAG3245800.065.3839733
TATGCCG3227050.065.3133146
ACGATCA3255300.065.31023432
TGCCGTC3208200.065.2730448
CACGATC3251850.065.24081431
CCGTCTT3180350.065.2332250
GCCGTCT3182200.065.2317149
CGTCTTC3178850.065.21126651
GTCACGA3271750.065.1842229
ATCTCGT2787600.065.1651140
TCACGAT3273400.065.1330830
AGTCACG3312800.064.929628
CGTCTGA3392600.064.70827516
ACACGTC3412050.064.6131413
Produced by FastQC (version 0.11.5)