FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_mb226.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_mb226.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31444041
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC2257630.7179834169533108TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG308650.062.28949739
CTCGTAT313400.061.65806242
TCGTATG319600.061.5465143
TCTCGTA307400.061.5413141
CGTATGC339850.061.38071444
TATGCCG345550.060.5705646
ATGCCGT335050.060.50662247
TGCCGTC329700.059.74800548
ACACGTC455050.059.6436513
CGTCTGA454950.059.57234216
CGCGTAT32900.059.45773742
ACGTCTG459900.059.12109415
ATCTCGT323700.058.4853440
CACGTCT467650.058.2310314
TCGCGTA32000.058.17765441
GCCGTCT311450.058.170949
CCGTCTT319200.058.07528350
CGTCTTC313700.056.43845451
GATCGGA501700.055.009181
ATCGGAA504250.054.9926762
Produced by FastQC (version 0.11.5)