FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_mb225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_mb225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22397996
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1780910.7951202420073652TruSeq Adapter, Index 3 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233260.10414324567251464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG252750.063.8003143
CTCGTAT239750.063.7273142
CGTATGC262100.063.5814844
TCTCGTA226600.063.4411541
ATGCCGT259900.063.2451847
TATGCCG265650.062.586946
TGCCGTC263800.061.59421548
CCGTCTT260200.061.14315450
GCCGTCT261400.060.47340449
CATCTCG232750.060.2004939
CGTCTTC260450.060.1171451
CGTCTGA297050.059.96267716
ACGTCTG298500.059.9170515
ACACGTC299250.059.86056513
ATCTCGT236700.059.3144240
CACGTCT309300.058.00591314
GATCGGA318850.057.186761
ATCGGAA321150.057.0786362
GCACACG320650.055.9746411
CACACGT322100.055.75524512
Produced by FastQC (version 0.11.5)