FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_mb224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_mb224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25868735
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC5070441.9600649200666362TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATGC327650.12665868663465762TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC259010.10012472585149604TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA791000.065.842771
CTCGTAT695350.065.82146542
TCGTATG746300.065.63222543
TCTCGTA658550.065.6311341
CGTATGC756750.065.503844
ATCGGAA799700.065.414162
CGATCTC615900.065.3911338
CACGATC615400.065.34720636
ACGATCT613200.065.1998137
ATGCCGT752400.065.0885647
ATCACGA700600.065.0172934
ACACGTC790800.064.9861413
TATGCCG757350.064.9676146
TGCCGTC752150.064.9211948
ACGTCTG792700.064.8655715
CATCACG753600.064.84209433
TCACGAT665000.064.8197735
CGTCTGA791250.064.7596416
GCCGTCT746950.064.6711149
CCGTCTT747050.064.6029350
Produced by FastQC (version 0.11.5)