FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_mb223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_mb223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33738820
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC423510.12552602610286903TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG398540.11812505594445805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT83550.042.80484842
TCGTATG90050.042.24120343
CGTATGC92900.041.88776844
TCTCGTA84600.041.65312641
AATCTCG82800.041.37496639
ATGCCGT94650.040.70768747
TATGCCG96000.040.68143546
ATCTCGT92350.037.4754340
TGCCGTC105350.036.3412348
CCGTCTT108250.034.88344650
GCCGTCT110950.033.78185749
CGTCTTC121200.030.83881651
GTATGCC142150.027.49849945
GTAATCT155550.022.45132437
GCTTGAA187150.020.30938159
TGTAATC192650.018.78164536
CCTTGTA228450.018.05948433
TAATCTC200050.017.57972738
TGCTTGA218550.017.34299558
CTTGTAA230300.017.1546634
Produced by FastQC (version 0.11.5)