FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_mb222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_mb222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25888781
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1363100.5265215075209605TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG194050.061.1347743
CTCGTAT187600.060.73694642
TCTCGTA182250.060.59940341
CGTATGC209150.060.4360844
ATGCCGT209100.059.0444847
TATGCCG214350.058.96971546
ATCTCGT190300.057.7227840
TGCCGTC216550.056.5448548
ACACGTC264550.055.54194313
CGTCTGA263950.055.38989616
GCCGTCT208100.055.29313349
CCGTCTT211050.055.2009650
CATCTCG201550.055.03886839
ACGTCTG269400.054.39924215
GATCGGA277850.054.0346151
ATCGGAA279500.053.9883462
CACGTCT277350.052.89030514
CGTCTTC217550.052.6186451
GTATGCC243100.052.35569445
TCGGAAG298350.050.8818933
Produced by FastQC (version 0.11.5)