FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_mb221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_mb221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34068754
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC352980.10360813312984678TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG69750.041.190443
CGTATGC72600.039.38113444
CTCGTAT71950.039.2012642
TCTCGTA72000.039.17392341
TATGCCG75150.037.7659546
TATCTCG75800.036.8867339
ATGCCGT76750.036.61419747
ATCTCGT84550.033.5246340
TGCCGTC88500.031.5949148
CGCCAAT91400.031.54789733
CCGTCTT90900.030.2993150
GCCGTCT93650.029.67090849
CGTCTTC102000.027.51672751
ACGCCAA106550.027.09496732
GTCACGC120500.024.13262729
GTATGCC121600.023.59870545
TCACGCC127350.022.72451830
CACGCCA131550.021.8926231
AGTCACG135700.021.79064628
GCCAATA148700.019.95609534
Produced by FastQC (version 0.11.5)