FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_mb220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_mb220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30785353
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2823980.917312853290979TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC402600.1307764767225505TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG448200.063.48475643
CTCGTAT389000.063.30460442
CGTATGC470850.063.0998144
TCTCGTA371250.063.0884141
ATGCCGT469100.062.75425347
TATGCCG476300.062.51076546
TGCCGTC472850.061.10992448
GCCGTCT455900.060.77274349
CCGTCTT463750.060.280950
ATCTCGT368050.060.12838740
GATCTCG372850.059.94540439
CGTCTGA556100.059.93065316
ACGTCTG560300.059.51225315
ACACGTC561050.059.42646413
CGTCTTC465050.058.66047351
CACGTCT571150.058.43072514
GTATGCC514550.058.3195945
CACACGT598250.055.7955912
GTCGTAT69700.055.4787842
ATCGGAA621500.055.0684472
Produced by FastQC (version 0.11.5)