FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_mb219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_mb219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35008765
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG62250.035.47192843
CTCGTAT59400.033.87480542
CGTATGC68600.032.2400144
ATGCCGT68250.031.99606347
TATGCCG70750.031.40889246
TCTCGTA62350.031.14955941
AATCTCG62750.029.5568139
TGCCGTC81850.026.93636348
ATCTCGT71250.026.3252740
CCGTCTT85750.025.42638650
GCCGTCT86600.025.4187549
CGTCTTC96450.022.8959451
GTATGCC124000.018.23121345
GACCAAT152150.013.56970735
GCTTGAA170500.013.30138559
ACCAATC143550.012.94439436
CCAATCT174050.011.15857537
CAATCTC180000.011.00366738
TGCTTGA204650.010.97926458
ACTGACC211100.010.87440332
Produced by FastQC (version 0.11.5)