FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_mb218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_mb218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34145523
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3722671.0902366321933332TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG486750.064.97780643
CTCGTAT479950.064.69498442
CGATGTA530450.064.57786634
CGTATGC507500.064.4183444
TCTCGTA464350.064.4039341
ATGCCGT506000.064.0502347
TATCTCG469300.063.9928739
ACCGATG542550.063.9310732
TATGCCG513300.063.82007646
CACCGAT544500.063.70230531
CCGATGT547400.063.26874533
ATCTCGT475500.062.8053140
TGCCGTC511100.062.46018248
GCCGTCT494050.062.3495549
GTCACCG562550.062.00715329
CCGTCTT501800.061.98013350
TCACCGA565850.061.5214830
CGTCTGA588800.060.9061416
CGTCTTC504100.060.24637251
ACACGTC595950.060.20459713
Produced by FastQC (version 0.11.5)